MUTANOLYSIN
RECOMBINANT
Recombinant mutanolysin
A&A Biotechnology is proud to offer recombinant mutanolysin expressed in bacteria. We are first to produce recombinant version of this enzyme worldwide. Our recombinant mutanolysin exhibits superior purity and affordable price, compared to native alternative products available from other vendors. Enzyme is safe, being a product originating from organism recognized by U.S Food and Drug Administration as GRAS (Generally Recognized as Safe).
General description
Mutanolysin (EC 3.2.1.17) (N-acetylmuramidase) is a muralytic enzyme that cleaves the β-N-acetylmuramyl-(1→4)-N-acetylglucosamine linkage of the bacterial cell wall peptidoglycan-polysaccharide. Its carboxy terminal moieties are involved in the recognition and binding of unique cell wall structures abundant in many gram-positive bacteria.
Application
- Mild conditions formation of spheroplasts of gram-positive bacteria
- Enzymatic cell lysis in DNA and RNA isolation process
- Effective lysis of gram-positive bacteria in environmental studies and DNA-based microbial detection
Recombinant mutanolysin effectively digests cell wall of particulary resistant to lysis bacteria.
Including but not limited to: Streptococcus, Lactobacillus, Lactococcus and Listeria.
Concentration
10 U/µl
Unit definition
One unit will produce a ∆A600 nm of 0.01 per minute at pH 6.0 at 37 °C in a 1 ml volume using suspension of Streptococcus faecalis cell wall as substrate.
Products
Mutanolysin, recombinant (solution)
Mutanolysin, recombinant (lyophilisate)
Bulk quantities available upon request. Contact us at: info@aabiot.com
Mutanolysin is also available as a part of our dedicated kits for genomic DNA isolation:
Genomic Mini AX Bacteria+ SPIN, cat#. 060-100MS
Genomic Micro AX Bacteria+ GRAVITY, cat#. 102-100M
Genomic Mini AX Bacteria+, cat#. 060-60M
Ordering
free samples available!
orders@aabiot.com, +48 58 6982194
visit us at www.aabiot.com
Recombinant mutanolysin and lysozyme synergism
Mutanolysin lytic activity may differ substantially when lysing various, even closely related, bacteria. The lysis effectiveness depends on peptidoglycan cell wall structure and bacteria growth conditions. It is especially important issue to be considered when processing environmental samples. For some hard-to-lyse microorganisms the application of mutanolysin and lysozyme mixture may drastically increase the DNA/RNA yield. Please see below the synergistic effect of lytic activities of recombinant mutanolysin and lysozyme mixture.
For supplementing lysozyme application we recommend Lysozyme (cat. # 1005-10 or 1005-50).
Al. Zwycięstwa 96/98, 81-451 Gdynia, Poland
phone +48 58 6982194, fax +48 58 6228578
info@aabiot.com, www.aabiot.com